GJA5/Connexin 40 Polyclonal antibody

GJA5/Connexin 40 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 26602-1-AP

Synonyms

Connexin 40, GJA5, GJA 5, Gap junction alpha-5 protein, Gap junction alpha 5 protein

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Validation Data Gallery

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  IHC staining of mouse lung using 26602-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26602-1-AP (GJA5/Connexin 40 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 26602-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26602-1-AP (Connexin 40 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26602-1-AP targets GJA5/Connexin 40 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GJA5/Connexin 40 fusion protein Ag24266
• Full Name: gap junction protein, alpha 5, 40kDa
• Calculated Molecular Weight: 40 kDa
• GenBank Accession Number: BC013313
• Gene Symbol: GJA5
• Gene ID (NCBI): 2702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Connexin 40 (Cx40), also known as Gap junction alpha 5 protein (GJA5), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Four connexins (Cxs) are expressed in the cardiovascular system (Cx37, Cx40, Cx43, and Cx45) and are involved in the development of the cardiovascular system(PMID: 37331352; PMID: 17922338).