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GIT1 Polyclonal antibody

GIT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26247-1-AP

Synonyms

Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, CAT 1, ARF GTPase-activating protein GIT1

Validation Data Gallery

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  • Western Blot (WB) analysis of ROS1728 cells using GIT1 Polyclonal antibody (26247-1-AP)

    WB analysis of ROS1728 using 26247-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 26247-1-AP (GIT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inROS1728 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26247-1-AP targets GIT1 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GIT1 fusion protein Ag24648
Full Name G protein-coupled receptor kinase interacting ArfGAP 1
Calculated Molecular Weight 694 aa, 77 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC067358
Gene Symbol GIT1
Gene ID (NCBI) 28964
RRIDAB_2880445
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GIT1 (ARF GTPase-activating protein GIT1) is a member of the GIT subfamily of ADP ribosylation factor (ARF)-GTPase activating protein (GAP) family of proteins, which are characterized by an ARFGAP domain that stimulates the GTPase activity of proteins. GIT1 was highly expressed in the nervous system, and its expression was maintained throughout all brain neuritogenesis stages (PMID: 27127481).

Protocols

Product Specific Protocols
WB protocol for GIT1 antibody 26247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Sammy (Verified Customer) (11-20-2023)

Detects and band above the expected kDa but has been validated by knock down. Antibody also works by immunofluorescence where it detects GIT1 at focal adhesions.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 1000
  • Cell Tissue Type: HUVEC
GIT1 Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:26247-1-AP)