GIP Polyclonal antibody

GIP Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 18034-1-AP

Synonyms

gastric inhibitory polypeptide, GIP

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Validation Data Gallery

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  IHC staining of human pancreas using 18034-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 18034-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat pancreas using 18034-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 18034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 18034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 18034-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 18034-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 18034-1-AP (GIP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 18034-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 18034-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18034-1-AP (GIP Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human pancreas tissue, rat pancreas tissue, mouse pancreas tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18034-1-AP is GIP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GIP fusion protein Ag12634
• Full Name: gastric inhibitory polypeptide
• Calculated Molecular Weight: 153 aa, 17 kDa
• GenBank Accession Number: BC069663
• Gene Symbol: GIP
• Gene ID (NCBI): 2695
• RRID: AB_2878484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GIP is an incretin hormone and belongs to the glucagon superfamily. GIP is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption.GIP stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shashi (Verified Customer) (01-30-2020)

worked beautifully

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human small intestine Tissue