PSF3 Polyclonal antibody

• 

  WB analysis of HeLa cells using 16345-1-AP

  WB result from Andre Franz in Hela cell of 16345-1-AP (anti-GINS3).

• 

  WB analysis of MCF-7 using 16345-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16345-1-AP (GINS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16345-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16345-1-AP (GINS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 16345-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16345-1-AP (PSF3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16345-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16345-1-AP (GINS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PSF3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16345-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GINS3, GINS complex subunit 3

---

Tested Applications

• Positive WB detected in: MCF7 cells, HeLa cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16345-1-AP targets PSF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSF3 fusion protein Ag9534
• Full Name: GINS complex subunit 3 (Psf3 homolog)
• Calculated Molecular Weight: 216 aa, 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC014437
• Gene Symbol: GINS3
• Gene ID (NCBI): 64785
• RRID: AB_10804660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BRX5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.