GINS1 Polyclonal antibody

GINS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16061-1-AP

Synonyms

PSF1, KIAA0186, GINS complex subunit 1

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Validation Data Gallery

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  WB analysis using 16061-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16061-1-AP (GINS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 16061-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16061-1-AP (GINS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, SH-SY5Y cells, U-251 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16061-1-AP targets GINS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GINS1 fusion protein Ag8895
• Full Name: GINS complex subunit 1 (Psf1 homolog)
• Calculated Molecular Weight: 196 aa, 23 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC012542
• Gene Symbol: GINS1
• Gene ID (NCBI): 9837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GINS1, also known as Partner of SLD Five 1 (PSF1) is a member of the GINS (Go-Ichi-Nii-San) complex, which plays a vital role in the initiation and elongation of DNA replication (PMID: 34414190). GINS1 was initially found to be predominantly expressed in highly proliferating cells instead of mature cells. Later on, it was found that GINS was involved in the tumorigenesis of various cancers, including breast cancer, colorectal cancer, and hepatocellular carcinoma (PMID: 36065190).