GIMAP5 Polyclonal antibody

GIMAP5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11068-1-AP

Synonyms

GIMAP5, GTPase IMAP family member 5, GTPase, IMAP family member 5, hIAN5, HIMAP3, IAN 5, IAN4, IAN4L1, IAN5, IMAP3, Immunity associated protein 3

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Validation Data Gallery

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  WB analysis of Jurkat using 11068-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11068-1-AP (GIMAP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 11068-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11068-1-AP (GIMAP5 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

11068-1-AP targets GIMAP5 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GIMAP5 fusion protein Ag1509
• Full Name: GTPase, IMAP family member 5
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC011732
• Gene Symbol: GIMAP5
• Gene ID (NCBI): 55340
• RRID: AB_2111880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GIMAP5 gene encodes immunity-associated nucleotide 5 protein (IAN5) belonging to GTPase immune-associated protein subfamily. GIMAP5 is widely expressed with high levels in lymph node and spleen. The loss of Gimap5 (GTPase of the 5) gene function is the underlying cause of lymphopenia and autoimmune diabetes in the BioBreeding (BB) rat. Mouse gimap5 is necessary for the survival of peripheral T cells, NK/NKT-cell development, hematopoietic progenitor cell survival and the maintenance of normal liver function. GIMAP5 may regulate T lymphocyte survival by mechanisms that operate upstream of mitochondria.

Publications

Species	Application	Title
human	WB	Mol Cancer; Identification of novel Notch target genes in T cell leukaemia.; Authors - Chadwick Nicholas N
human	WB	Blood Cells Mol Dis; Notch protection against apoptosis in T-ALL cells mediated by GIMAP5.; Authors - Chadwick Nicholas N