GILZ Polyclonal antibody, PBS Only

GILZ Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12352-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse brain using 12352-1-AP (same clone as 12352-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12352-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12352-1-AP (same clone as 12352-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12352-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12352-1-AP (same clone as 12352-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12352-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12352-1-AP (same clone as 12352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12352-1-AP (GILZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12352-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 12352-1-AP (same clone as 12352-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using GILZ antibody (12352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12352-1-PBS in a different storage buffer formulation.

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Product Information

12352-1-PBS targets GILZ in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GILZ fusion protein Ag3015
• Full Name: TSC22 domain family, member 3
• Calculated Molecular Weight: 134 aa, 15 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC018148
• Gene Symbol: GILZ
• Gene ID (NCBI): 1831
• RRID: AB_2272196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GILZ, also known as TSC22D3 and DSIPI, is a glucocorticoid responsive protein and is thought to mediate some of the anti-inflammatory effects of glucocorticoid receptors (GRs). GILZ is mainly localized in the cytoplasm. It is an anti-inflammatory molecule that could mediate the immunomodulatory activities of GCs and also plays a role in cell differentiation in various cells and tissues (PMID: 35011684).