GHITM Recombinant antibody, PBS Only (Detector)

GHITM Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240541D3

Cat no : 83548-3-PBS

Synonyms

My021, MICS1, Growth hormone-inducible transmembrane protein, DERP2, Dermal papilla-derived protein 2

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Validation Data Gallery

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  WB analysis using 83548-3-RR (same clone as 83548-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83548-3-RR (GHITM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

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  WB analysis using 83548-3-RR (same clone as 83548-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83548-3-RR (GHITM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83548-3-RR (same clone as 83548-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GHITM Recombinant antibody (83548-3-RR, Clone:240541D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00524-1

  Cytometric bead array standard curve of MP00524-1, GHITM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83548-2-PBS. Detection antibody: 83548-3-PBS. Standard: Ag35170. Range: 0.78-100 ng/mL.

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  Cytometric bead array standard curve of MP00524-2

  Cytometric bead array standard curve of MP00524-2, GHITM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83548-1-PBS. Detection antibody: 83548-3-PBS. Standard: Ag35170. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83548-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83548-3-RR against Human GHITM were performed. The affinity constant is 2.41 nM.

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Product Information

83548-3-PBS targets GHITM as part of a matched antibody pair:

MP00524-1: 83548-2-PBS capture and 83548-3-PBS detection (validated in Cytometric bead array)

MP00524-2: 83548-1-PBS capture and 83548-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GHITM fusion protein Ag35170
• Full Name: growth hormone inducible transmembrane protein
• Calculated Molecular Weight: 345 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC010354
• Gene Symbol: GHITM
• Gene ID (NCBI): 27069
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GHITM, also known as MICS1, TMBIM5 or DERP2, is a mitochondrial protein that localizes in the inner membrane. GHITM is involved in mitochondrial morphology in specific cristae structures and the apoptotic release of cytochrome c from the mitochondria (PMID: 18417609). The gene of GHITM maps to chromosome 10q23.1, and encodes a 345-amino-acid protein with a calculated molecular mass of 37 kDa.