GH1 Recombinant antibody

GH1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4G8

Cat no : 82813-2-RR

Synonyms

GH, 4G8, growth hormone 1, Growth hormone, GH-N

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Validation Data Gallery

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  WB analysis using 82813-2-RR

  Human placenta tissue was subjected to SDS PAGE followed by western blot with 82813-2-RR (GH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 82813-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 82813-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82813-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82813-2-RR against Human GH1 were performed. The affinity constant is 0.76 nM.

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Tested Applications

• Positive WB detected in: human saliva
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82813-2-RR targets GH1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: GH1
• Calculated Molecular Weight: 217 aa, 25 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC075012
• Gene Symbol: GH1
• Gene ID (NCBI): 2688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GH1, also named as GH and GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.