GH1 Polyclonal antibody

GH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55243-1-AP

Synonyms

GH, GH N, GH1, GHN, hGH N, Somatotropin

---

Validation Data Gallery

• 

  WB analysis of human placenta using 55243-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55243-1-AP (GH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 55243-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55243-1-AP (GH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 55243-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55243-1-AP (GH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pituitary using 55243-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pituitary using 55243-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pituitary adenoma using 55243-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pituitary adenoma using 55243-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human pituitary tissue, human pituitary adenoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

55243-1-AP targets GH1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GH1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: NM_000515
• Gene Symbol: GH1
• Gene ID (NCBI): 2688
• RRID: AB_11182710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GH1, also named as GH and GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. This antibody has no cross reaction to CSH1.

Publications

Species	Application	Title
rat	IF	Metabolism; PMID: 31666193; Authors - Jian Mao
mouse	WB,IF	Biochem Biophys Res Commun; Elongator regulates the melanocortin satiety pathway.; Authors - Joseph Walters
rat	WB	Toxicol Lett; The disrupting effect of chlormequat chloride on growth hormone is associated with pregnancy; Authors - Zongzhen Wu