GGH Polyclonal antibody

GGH Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13264-1-AP

Synonyms

Conjugase, Gamma Glu X carboxypeptidase, Gamma glutamyl hydrolase, GGH, GH

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Validation Data Gallery

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  WB analysis using 13264-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13264-1-AP (GGH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13264-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13264-1-AP (GGH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13264-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13264-1-AP (GGH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13264-1-AP

  IP result of anti-GGH (IP:13264-1-AP, 4ug; Detection:13264-1-AP 1:500) with HepG2 cells lysate 5600 ug.

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  IHC staining of human kidney using 13264-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13264-1-AP (GGH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13264-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13264-1-AP (GGH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, MCF-7 cells, HepG2 cells, HT-1080 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13264-1-AP targets GGH in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GGH fusion protein Ag3939
• Full Name: gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
• Calculated Molecular Weight: 318 aa, 36 kDa
• Observed Molecular Weight: 30-37 kDa, 55 kDa
• GenBank Accession Number: BC025025
• Gene Symbol: GGH
• Gene ID (NCBI): 8836
• RRID: AB_2877931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GGH (Gamma glutamyl hydrolase), also named as GH or Conjugase, is a key lysosomal enzyme involved in the metabolism of folic acid and in the action of antifolate drugs (PMID: 16945597). GGH catalyzes the removal of γ-linked polyglutamates from the intracellular folylpolyglutamates to yield folylmonoglutamate coenzymes (PMID: 9614206). The full-length protein has a calculated molecular mass of 36 kDa, contains four potential asparagine glycosylation sites, and was predicted to have a 24-amino-acid signal peptide (PMID: 8816764). GGH can form homodimer which contains two potential active sites (PMID: 16945597). Some bands can be detected by SDS-PAGE: 35-37kDa (full-length), 55kDa (glycosylated form) and 30-33kDa (signal peptide removed) (PMID: 8621474).

Publications

Species	Application	Title
human	WB	J Clin Invest; Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer; Authors - Xiaochao Tan
	WB	Front Pharmacol; Up-regulation of ABCG1 is associated with methotrexate resistance in acute lymphoblastic leukemia cells; Authors - Yao Chen
human	IHC,IF	Reprod Sci; The Identification of Gamma-Glutamyl Hydrolase in Uterine Corpus Endometrial Carcinoma: a Predictive Model and Machine Learning; Authors - Cheng Zhu