GGA2 Polyclonal antibody

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• KD/KO Validated

GGA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10356-1-AP

Synonyms

VHS domain and ear domain of gamma-adaptin, Vear, Golgi-localized, gamma ear-containing, ARF-binding protein 2, Gamma-adaptin-related protein 2, ADP-ribosylation factor-binding protein GGA2

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Validation Data Gallery

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  WB analysis of HeLa using 10356-1-AP

  WB result of GGA2 antibody (10356-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GGA2 transfected HeLa cells.

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  WB analysis of mouse brain using 10356-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 10356-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse adipose using 10356-1-AP

  mouse adipose tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10356-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10356-1-AP (GGA2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10356-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10356-1-AP (GGA2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10356-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GGA2 antibody (10356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, mouse adipose tissue, human kidney tissue
• Positive IHC detected in: human colon cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

10356-1-AP targets GGA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GGA2 fusion protein Ag0370
• Full Name: golgi associated, gamma adaptin ear containing, ARF binding protein 2
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC000284
• Gene Symbol: GGA2
• Gene ID (NCBI): 23062
• RRID: AB_2110735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GGA2, also known as VEAR, belongs to the GGA protein family and regulates the trafficking of proteins between the trans-Golgi network and the lysosome. GGA2 has an amino-terminal VHS domain in its N terminus which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.

Publications

Species	Application	Title
human	WB	J Cell Biol; Cargo-selective SNX-BAR proteins mediate retromer trimer independent retrograde transport.; Authors - Arunas Kvainickas
rat	WB	Front Cardiovasc Med; Modular Screening Reveals Driver Induced Additive Mechanisms of Baicalin and Jasminoidin on Cerebral Ischemia Therapy.; Authors - Bing Li