GFM1 Polyclonal antibody

GFM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 14274-1-AP

Synonyms

EGF1, EF-Gmt, EFGM, EFG1, EF Gmt

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Validation Data Gallery

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  WB analysis of mouse kidney using 14274-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14274-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14274-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14274-1-AP

  IP result of anti-GFM1 (IP:14274-1-AP, 4ug; Detection:14274-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human kidney using 14274-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14274-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 14274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14274-1-AP (GFM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HeLa cells, human heart tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• CoIP: See 1 publications below

Product Information

14274-1-AP targets GFM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GFM1 fusion protein Ag5616
• Full Name: G elongation factor, mitochondrial 1
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC049210
• Gene Symbol: GFM1
• Gene ID (NCBI): 85476
• RRID: AB_2110140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Different factors catalyze the three stages of protein translation: initiation, elongation, and termination. There are two translational systems in eukaryotes, one in the cytoplasm and the other in the mitochondria. In mitochondria, the elongation phase requires three elongation factors (EF): Tu (TUFM), Ts (TSFM), and G (GFM1)[PMID:19716793]. GFM1 catalyzes translocation during peptide elongation and mediates ribosomal disassembly during ribosome recycling in concert with the ribosomal recycling factor (RRF). [PMID:16487710]

Publications

Species	Application	Title
human	WB	Sci Transl Med; Molecular diagnosis of infantile mitochondrial disease with targeted next-generation sequencing.; Authors - Sarah E Calvo
mouse	WB,CoIP	Int J Mol Sci; Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling; Authors - Jana Key
human,mouse	WB	Cells; Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.; Authors - Jana Key
human	WB	Mitochondrion; Toward genotype phenotype correlations in GFM1 mutations.; Authors - Galmiche Louise L

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Miguel (Verified Customer) (03-21-2022)

WB analysis of mouse liver using 14274-1-AP. Mouse liver homogenates in Ripa buffer were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:1000 incubated at 4°C O/N. KI/KO: Compound heterozygous knock-in/knock-out (Gfm1R671C/-) mouse model (1). WT: Wild type control mice (Gfm1+/+). (1) ) Molina-Berenguer M, Vila-Julià F, Pérez-Ramos S, Salcedo-Allende MT, Cámara Y, Torres-Torronteras J, Martí R. Dysfunctional mitochondrial translation and combined oxidative phosphorylation deficiency in a mouse model of hepatoencephalopathy due to Gfm1 mutations. FASEB J. 2022 Jan;36(1):e22091. doi: 10.1096/fj.202100819RRR. PMID: 34919756.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver homogenate

GFM1 Antibody Western Blot validation (1:1000 dilution) in Mouse liver homogenate (Cat no:14274-1-AP)