GEN1 Polyclonal antibody

GEN1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29617-1-AP

Synonyms

DKFZp781F0986, FLJ40869, Gen, GEN1

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Validation Data Gallery

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  WB analysis using 29617-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29617-1-AP (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HL-60 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29617-1-AP targets GEN1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GEN1 fusion protein Ag31227
• Full Name: Gen homolog 1, endonuclease (Drosophila)
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_182625
• Gene Symbol: GEN1
• Gene ID (NCBI): 348654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GEN1 is a endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. GEN1 efficiently cleaves both single and double HJs contained within large recombination intermediates.