GEFT Polyclonal antibody, PBS Only

• 

  WB analysis of human brain using 17028-1-AP (same clone as 17028-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 17028-1-AP (GEFT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17028-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 17028-1-AP (same clone as 17028-1-PBS)

  IP result of anti-GEFT (IP:17028-1-AP, 4ug; Detection:17028-1-AP 1:400) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 17028-1-PBS in a different storage buffer formulation.

GEFT Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 17028-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

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Product Information

17028-1-PBS targets GEFT in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GEFT fusion protein Ag10436
• Full Name: RhoA/RAC/CDC42 exchange factor
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC012860
• Gene Symbol: GEFT
• Gene ID (NCBI): 115557
• RRID: AB_2279083
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86VW2
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68 kDa).