GDPD5 Polyclonal antibody

• Featured Product
• KD/KO Validated

GDPD5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25703-1-AP

Synonyms

Phosphoinositide phospholipase C GDPD5, Glycerophosphodiester phosphodiesterase domain-containing protein 5, Glycerophosphocholine phosphodiesterase GDPD5, GDE2, EC:3.1.4.2

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Validation Data Gallery

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  WB analysis of K-562 using 25703-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25703-1-AP (GDPD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 25703-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25703-1-AP (GDPD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 25703-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25703-1-AP (GDPD5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 25703-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25703-1-AP (GDPD5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, COLO 320 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• CoIP: See 1 publications below

Product Information

25703-1-AP targets GDPD5 in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDPD5 fusion protein Ag22530
• Full Name: glycerophosphodiester phosphodiesterase domain containing 5
• Calculated Molecular Weight: 605 aa, 69 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC018771
• Gene Symbol: GDPD5
• Gene ID (NCBI): 81544
• RRID: AB_2880200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDPD5(Glycerophosphodiester phosphodiesterase domain-containing protein 5) is also named as GDE2 and belongs to the glycerophosphoryl diester phosphodiesterase family. The deduced sequence of GDE2 contains 607 amino acids with seven putative transmembrane regions. GDPD5 is a glycerophosphocholine phosphodiesterase that osmotically regulates the osmoprotective organic osmolyte GPC(PMID:18667693). It has 5 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,CoIP	Cancer Res; PRKDC Induces Chemoresistance in Osteosarcoma by Recruiting GDE2 to Stabilize GNAS and Activate AKT; Authors - Wenchao Zhang ; KD Validated
human	WB	J Biol Chem; Persistent ER stress causes GPI anchor deficit to convert a GPI-anchored prion protein (PrP) into pro-PrP via the ATF6-miR449c-5p-PIGV axis; Authors - JingFeng Li