GDPD5 Polyclonal antibody

GDPD5 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16494-1-AP

Synonyms

GDE2, GDPD5, PP1665, PP9363, UNQ1850/PRO3580

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Validation Data Gallery

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  IP experiment of mouse skeletal muscle using 16494-1-AP

  IP result of anti-GDPD5 (IP:16494-1-AP, 4ug; Detection:16494-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human spleen using 16494-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 16494-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 16494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16494-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16494-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16494-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16494-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16494-1-AP (GDPD5 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human spleen tissue, human kidney tissue, human ovary tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16494-1-AP targets GDPD5 in IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDPD5 fusion protein Ag9622
• Full Name: glycerophosphodiester phosphodiesterase domain containing 5
• Calculated Molecular Weight: 127 aa, 13 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC020203
• Gene Symbol: GDPD5
• Gene ID (NCBI): 81544
• RRID: AB_2111683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDPD5(Glycerophosphodiester phosphodiesterase domain-containing protein 5) is also named as GDE2 and belongs to the glycerophosphoryl diester phosphodiesterase family. The deduced sequence of GDE2 contains 607 amino acids with seven putative transmembrane regions. GDPD5 is a glycerophosphocholine phosphodiesterase that osmotically regulates the osmoprotective organic osmolyte GPC(PMID:18667693). It has 5 isoforms produced by alternative splicing.