GDPD2 Polyclonal antibody

GDPD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25074-1-AP

Synonyms

GDE3, GDPD2, OBDPF

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Validation Data Gallery

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  WB analysis of mouse skin using 25074-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 25074-1-AP (GDPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 25074-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25074-1-AP (GDPD2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 25074-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25074-1-AP (GDPD2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skin tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25074-1-AP is GDPD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDPD2 fusion protein Ag18377
• Full Name: glycerophosphodiester phosphodiesterase domain containing 2
• Calculated Molecular Weight: 539 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC032009
• Gene Symbol: GDPD2
• Gene ID (NCBI): 54857
• RRID: AB_2879885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDPD2(Glycerophosphoinositol inositolphosphodiesterase) is also named as GDE3, OBDPF and belongs to the glycerophosphoryl diester phosphodiesterase family. It has glycerophosphoinositol inositolphosphodiesterase activity and specifically hydrolyzes glycerophosphoinositol, with no activity for other substrates such as glycerophosphoinositol 4-phosphate, glycerophosphocholine, glycerophosphoethanolamine, and glycerophosphoserine. This protein has 3 isoforms produced by alternative splicing.