GDNF Recombinant antibody

GDNF Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230358G6

Cat no : 83087-2-RR

Synonyms

Glial cell line-derived neurotrophic factor, ATF2, ATF1, ATF, Astrocyte-derived trophic factor

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Validation Data Gallery

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  WB analysis of mouse brain using 83087-2-RR

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 83087-2-RR (GDNF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83087-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83087-2-RR against Human GDNF were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83087-2-RR is GDNF Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GDNF fusion protein Ag24068
• Full Name: glial cell derived neurotrophic factor
• Calculated Molecular Weight: 211 aa, 24 kDa
• Observed Molecular Weight: 24-30 kDa
• GenBank Accession Number: BC069369
• Gene Symbol: GDNF
• Gene ID (NCBI): 2668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glial-derived neurotrophic factor (GDNF) is a member of the transforming growth factor (TGF)-α family and exhibits potent neuroprotective activity. GDNF is a potent promoter of neuronal survival in the CNS and peripheral nervous system (PNS). It has a relatively high speciicity for dopaminergic neurons and thus become an efective treatment for Parkinson's diseases (PD), with clinical trials currently in progress.