GDI2 Polyclonal antibody

GDI2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 55096-1-AP

Synonyms

GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB

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Validation Data Gallery

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  IHC staining of human skin using 55096-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55096-1-AP (GDI2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 55096-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55096-1-AP (GDI2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 55096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55096-1-AP (GDI2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 55096-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55096-1-AP (GDI2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human skin tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55096-1-AP targets GDI2 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GDP dissociation inhibitor 2
• Calculated Molecular Weight: 51 kDa
• GenBank Accession Number: NM_001494
• Gene Symbol: GDI2
• Gene ID (NCBI): 2665
• RRID: AB_11183032
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). The antibody is specific to GDI2. And it has no cross reaction to GDI1.