GDI1 Polyclonal antibody

GDI1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10249-1-AP

Synonyms

GDI 1, GDI1, GDIL, GDP dissociation inhibitor 1, MRX41, MRX48, Oligophrenin 2, OPHN2, Protein XAP 4, Rab GDI alpha, RABGD1A, RABGDIA, XAP 4, XAP4

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Validation Data Gallery

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  WB analysis of mouse brain using 10249-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10249-1-AP (GDI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10249-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10249-1-AP (GDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10249-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10249-1-AP (GDI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10249-1-AP

  IP result of anti-GDI1 (IP:10249-1-AP, 4ug; Detection:10249-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 10249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10249-1-AP (GDI1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10249-1-AP (GDI1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10249-1-AP (GDI1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10249-1-AP (GDI1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 4 publications below
• ELISA: See 1 publications below

Product Information

10249-1-AP targets GDI1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDI1 fusion protein Ag0288
• Full Name: GDP dissociation inhibitor 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC000317
• Gene Symbol: GDI1
• Gene ID (NCBI): 2664
• RRID: AB_2111520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55kda protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034) This antibody can bind both GDIs for the close sequences.

Publications

Species	Application	Title
human	WB,IF	Neurology; Antibodies in patients with neuropsychiatric systemic lupus erythematosus.; Authors - Kimura A A
mouse	IF	EMBO Rep; Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.; Authors - Ye Chen
mouse	WB	Neurobiol Dis; Disruption of Rab11 activity in a knock-in mouse model of Huntington's disease.; Authors - Xueyi Li
human,mouse	ELISA	Proteomics; 2-D DIGE analysis implicates cytoskeletal abnormalities in psychiatric disease.; Authors - English Jane A JA
mouse	IF	Int J Mol Sci; Poly(ADP-ribose) Polymerase 1 Mediates Rab5 Inactivation after DNA Damage; Authors - Masato Mashimo
human	IF	Nature; A cross-species proteomic map reveals neoteny of human synapse development; Authors - Li Wang