GDF9 Polyclonal antibody

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  WB analysis using 29309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29309-1-AP (GDF9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 29309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29309-1-AP (GDF9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SKOV-3 using 29309-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using GDF9 antibody (29309-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

GDF9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 29309-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GDF-9, GDF 9

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Tested Applications

• Positive WB detected in: A2780 cells, SKOV-3 cells, mouse ovary tissue, rat ovary tissue
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29309-1-AP targets GDF9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDF9 fusion protein Ag29603
• Full Name: growth differentiation factor 9
• Calculated Molecular Weight: 454 aa, 51 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC096228
• Gene Symbol: GDF9
• Gene ID (NCBI): 2661
• RRID: AB_3086112
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60383
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDF9 (Growth/differentiation factor 9) belongs to the TGF-beta family. GDF9 is required for ovarian folliculogenesis. GDF9 and BMP15 are oocyte-secreted factors with a leading role in the control of ovarian function in female reproduction, modulating both the cell fate of the somatic granulosa cells and the quality and developmental competence of the egg (PMID: 29544636). GDF9 promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation (PMID: 19366876). It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. GDF9 attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B (PMID: 21829661). It suppresses FST and FSTL3 production in granulosa-lutein cells  (PMID: 21632818, PMID: 21829661).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yiran (Verified Customer) (09-19-2024)

Works well on paraffin-fixed sheep ovary for immunofluorescent

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Ovary

GDF9 Antibody Immunohistochemistry validation (1:100 dilution) in Ovary (Cat no:29309-1-AP)