GDF-15 Polyclonal antibody

• Featured Product
• KD/KO Validated

GDF-15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

32

Cat no : 27455-1-AP

Synonyms

GDF15, GDF 15, NAG-1, MIC-1, Growth/differentiation factor 15

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Validation Data Gallery

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  WB analysis using 27455-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27455-1-AP (GDF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27455-1-AP

  WB result of GDF15 antibody (27455-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GDF15 transfected HepG2 cells.

• 

  WB analysis using 27455-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 27455-1-AP (GDF15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 27455-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27455-1-AP (GDF15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 27455-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27455-1-AP (GDF15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 27455-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27455-1-AP (GDF15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HepG2 cells, human placenta tissue, LNCaP cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 28 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Product Information

27455-1-AP targets GDF-15 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDF-15 fusion protein Ag26760
• Full Name: growth differentiation factor 15
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC008962
• Gene Symbol: GDF15
• Gene ID (NCBI): 9518
• RRID: AB_2880875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.

Publications

Species	Application	Title
human,mouse	WB	Gastroenterology; Hepatic Stellate Cells in Hepatocellular Carcinoma Promote Tumor Growth Via Growth Differentiation Factor 15 Production.; Authors - Yuta Myojin ; KO Validated
human	WB	Nat Commun; Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer.; Authors - Tiziano Bernasocchi
human,mouse	WB,IHC	Cancer Lett; SULF2 enhances GDF15-SMAD axis to facilitate the initiation and progression of pancreatic cancer.; Authors - Ruizhe He
rat	WB,IF	Oxid Med Cell Longev; Cyclic Polypeptide D7 Protects Bone Marrow Mesenchymal Cells and Promotes Chondrogenesis during Osteonecrosis of the Femoral Head via Growth Differentiation Factor 15-Mediated Redox Signaling.; Authors - Jiazheng Chen
human	WB	Cancers (Basel); GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.; Authors - Chiara Bellio ; KD Validated
human	WB	Mol Neurobiol; Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation; Authors - Ruicheng Yang