GDEP Polyclonal antibody

GDEP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22744-1-AP

Synonyms

GDEP, PCAN1

---

Validation Data Gallery

• 

  WB analysis of PC-3 using 22744-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22744-1-AP (GDEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 22744-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 22744-1-AP (GDEP Antibody) at dilution of 1:50. (under 10x lens).

• 

  IHC staining of human prostate cancer using 22744-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 22744-1-AP (GDEP Antibody) at dilution of 1:50. (under 40x lens).

• 

  IHC staining of human prostate cancer using 22744-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22744-1-AP (GDEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 22744-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia slide using 22744-1-AP (GDEP Antibody) at dilution of 1:50. (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 22744-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia slide using 22744-1-AP (GDEP Antibody) at dilution of 1:50. (under 40x lens).

• 

  IF Staining of PC-3 using 22744-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 22744-1-AP (GDEP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IHC detected in: human prostate cancer tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22744-1-AP is GDEP Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDEP fusion protein Ag19087
• Full Name: gene differentially expressed in prostate
• Calculated Molecular Weight: 34 aa, 4 kDa
• Observed Molecular Weight: 4 kDa
• GenBank Accession Number: BC125139
• Gene Symbol: GDEP
• Gene ID (NCBI): 118425
• RRID: AB_2879161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.