GDAP2 Polyclonal antibody

GDAP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17246-1-AP

Synonyms

Ganglioside-induced differentiation-associated protein 2

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Validation Data Gallery

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  WB analysis of Raji using 17246-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17246-1-AP (GDAP2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  IHC staining of human stomach using 17246-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17246-1-AP (GDAP2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 17246-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17246-1-AP (GDAP2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Raji cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17246-1-AP targets GDAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDAP2 fusion protein Ag11175
• Full Name: ganglioside induced differentiation associated protein 2
• Calculated Molecular Weight: 496 aa, 56 kDa
• Observed Molecular Weight: 56-60 kDa
• GenBank Accession Number: BC013132
• Gene Symbol: GDAP2
• Gene ID (NCBI): 54834
• RRID: AB_2878369
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.