GDAP1L1 Polyclonal antibody

GDAP1L1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11977-1-AP

Synonyms

GDAP1 L1, GDAP1L1

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Validation Data Gallery

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  WB analysis of mouse brain using 11977-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11977-1-AP (GDAP1L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 11977-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11977-1-AP (GDAP1L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 11977-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11977-1-AP (GDAP1L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11977-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11977-1-AP (GDAP1L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11977-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11977-1-AP (GDAP1L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, Y79 cells, fetal human brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11977-1-AP targets GDAP1L1 in WB, IHC, ELISA applications and shows reactivity with mouse, human samples.

• Tested Reactivity: mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDAP1L1 fusion protein Ag2602
• Full Name: ganglioside-induced differentiation-associated protein 1-like 1
• Calculated Molecular Weight: 367 aa, 42 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC009014
• Gene Symbol: GDAP1L1
• Gene ID (NCBI): 78997
• RRID: AB_2232105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.