GDAP1 Polyclonal antibody

GDAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13152-1-AP

Synonyms

CMTRIA, GDAP1

---

Validation Data Gallery

• 

  WB analysis using 13152-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 13152-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 13152-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of human brain using 13152-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: SH-SY5Y cells, human brain tissue, fetal human brain tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13152-1-AP targets GDAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDAP1 fusion protein Ag3769
• Full Name: ganglioside-induced differentiation-associated protein 1
• Calculated Molecular Weight: 358 aa, 41 kDa
• Observed Molecular Weight: 36-41 kDa
• GenBank Accession Number: BC024939
• Gene Symbol: GDAP1
• Gene ID (NCBI): 54332
• RRID: AB_2232103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDAP1 is a member of the ganglioside-induced differentiation-associated protein family, which may play a role in a signal transduction pathway during neuronal development. Mutations in this gene have been associated with various forms of Charcot-Marie-Tooth Disease and neuropathy.