GDA Polyclonal antibody

GDA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31493-1-AP

Synonyms

CYPIN, GAH, GDA, GUANASE, Guanine aminase, Guanine aminohydrolase, guanine deaminase, KIAA1258, NEDASIN, p51 nedasin

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Validation Data Gallery

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  WB analysis using 31493-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31493-1-AP (GDA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse kidney tissue, rat brain tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31493-1-AP targets GDA in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDA fusion protein Ag35661
• Full Name: guanine deaminase
• Observed Molecular Weight: 48-53 kDa
• GenBank Accession Number: BC053584
• Gene Symbol: GDA
• Gene ID (NCBI): 9615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDA ( guanine deaminase), also known as KIAA1258. The protein is enriched in the brain, intestine, kidney and liver. Cytoplasmic expression in the small intestine, hepatocytes, pancreatic islet cells and kidney tubules. This gene encodes an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. The molecular weight of GDA is 51 kDa, and it can recognize other isoforms.