GCSH Recombinant antibody

GCSH Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230505A3

Cat no : 83012-5-RR

Synonyms

GCE, GCSH, NKH

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Validation Data Gallery

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  WB analysis of HEK-293 using 83012-5-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 83012-5-RR (GCSH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83012-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83012-5-RR (GCSH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity experiments of 83012-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83012-5-RR against Human GCSH were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83012-5-RR is GCSH Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GCSH fusion protein Ag10174
• Full Name: glycine cleavage system protein H (aminomethyl carrier)
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: ~15 kDa
• GenBank Accession Number: BC000790
• Gene Symbol: GCSH
• Gene ID (NCBI): 2653
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encodes a precursor protein of 173 amino acids and a mature protein of 125 amino acids. The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).