GCSH Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00021-3

  Sandwich ELISA standard curve of MP00021-3, Human GCSH Recombinant Matched Antibody Pair - PBS only. 83012-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10174. 83012-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP00021-3

  The mean GCSH concentration was determined to be 24.39 ng/mL in HeLa cell extract based on a 3.60 mg/mL extract load and 23.77 ng/mL in HEK-293 cell extract based on a 3.40 mg/mL extract load.

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  Cytometric bead array standard curve of MP00021-3

  Cytometric bead array standard curve of MP00021-3, GCSH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83012-3-PBS. Detection antibody: 83012-2-PBS. Standard: Ag10174. Range: 0.625-20 ng/mL.

GCSH Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83012-3-PBS

Clone No.230505C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

NKH, MMDS7, Glycine cleavage system H protein, mitochondrial, GCE, 230505C10

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Product Information

83012-3-PBS targets GCSH as part of a matched antibody pair:

MP00021-3: 83012-3-PBS capture and 83012-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GCSH fusion protein Ag10174
• Full Name: glycine cleavage system protein H (aminomethyl carrier)
• Calculated Molecular Weight: 19 kDa
• GenBank Accession Number: BC000790
• Gene Symbol: GCSH
• Gene ID (NCBI): 2653
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P23434
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.