GCSH Polyclonal antibody

• Featured Product
• KD/KO Validated

GCSH Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 16726-1-AP

Synonyms

GCE, GCSH, NKH

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Validation Data Gallery

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  WB analysis of HEK-293 using 16726-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16726-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16726-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16726-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16726-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16726-1-AP

  IP Result of anti-GCSH (IP:16726-1-AP, 3ug; Detection:16726-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16726-1-AP (GCSH Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16726-1-AP (GCSH Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of rat ovary using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary slide using 16726-1-AP (GCSH Antibody) at dilution of 1:50.

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  IHC staining of rat ovary using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary slide using 16726-1-AP (GCSH Antibody) at dilution of 1:50.

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  IHC staining of human placenta using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 16726-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GCSH antibody 16726-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 16726-1-AP

  Immunofluorescent analysis of HepG2 cells, using GCSH antibody 16726-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human liver tissue, mouse brain tissue, mouse kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue, human placenta tissue, human testis tissue, rat ovary tissue, human skin tissue, human liver tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

16726-1-AP targets GCSH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GCSH fusion protein Ag10174
• Full Name: glycine cleavage system protein H (aminomethyl carrier)
• Calculated molecular weight: 19 kDa
• Observed molecular weight: 15 kDa
• GenBank accession number: BC000790
• Gene symbol: GCSH
• Gene ID (NCBI): 2653
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encoding a precursor protein of 173 amino acids and a mature protein of 125 amino acids.The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).

Publications

Species	Application	Title
		Science; Copper induces cell death by targeting lipoylated TCA cycle proteins.; Authors - Peter Tsvetkov
human	WB	Nat Commun; Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.; Authors - Jiliang Xia ; KD Validated
human	WB	J Biol Chem; Acute loss of iron-sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells.; Authors - Daniel R Crooks
human	WB,IHC,IF	Sci Rep; GCSH antisense regulation determines breast cancer cells' viability.; Authors - Anna Adamus
human	WB	J Biol Chem; FDX1 regulates cellular protein lipoylation through direct binding to LIAS; Authors - Margaret B Dreishpoon
human	WB	PLoS One; Amino Acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis.; Authors - Mark A Johnson