Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, human liver tissue, mouse brain tissue, mouse kidney tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human kidney tissue, human placenta tissue, human testis tissue, rat ovary tissue, human skin tissue, human liver tissue, human ovary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16726-1-AP targets GCSH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GCSH fusion protein Ag10174|
|Full Name||glycine cleavage system protein H (aminomethyl carrier)|
|Calculated molecular weight||19 kDa|
|Observed molecular weight||15 kDa|
|GenBank accession number||BC000790|
|Gene ID (NCBI)||2653|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encoding a precursor protein of 173 amino acids and a mature protein of 125 amino acids.The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).
|Product Specific Protocols|
|WB protocol for GCSH antibody 16726-1-AP||Download protocol|
|IHC protocol for GCSH antibody 16726-1-AP||Download protocol|
|IF protocol for GCSH antibody 16726-1-AP||Download protocol|
|IP protocol for GCSH antibody 16726-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Copper induces cell death by targeting lipoylated TCA cycle proteins.
Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.
J Biol Chem
Acute loss of iron-sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells.
GCSH antisense regulation determines breast cancer cells' viability.
J Biol Chem
FDX1 regulates cellular protein lipoylation through direct binding to LIAS
Amino Acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis.