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GCSH Polyclonal antibody
GCSH Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16726-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, human liver tissue, mouse brain tissue, mouse kidney tissue |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human kidney tissue, human placenta tissue, human testis tissue, rat ovary tissue, human skin tissue, human liver tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 10 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
Product Information
16726-1-AP targets GCSH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GCSH fusion protein Ag10174 |
Full Name | glycine cleavage system protein H (aminomethyl carrier) |
Calculated molecular weight | 19 kDa |
Observed molecular weight | 15 kDa |
GenBank accession number | BC000790 |
Gene symbol | GCSH |
Gene ID (NCBI) | 2653 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encoding a precursor protein of 173 amino acids and a mature protein of 125 amino acids.The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).
Protocols
Product Specific Protocols | |
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WB protocol for GCSH antibody 16726-1-AP | Download protocol |
IHC protocol for GCSH antibody 16726-1-AP | Download protocol |
IF protocol for GCSH antibody 16726-1-AP | Download protocol |
IP protocol for GCSH antibody 16726-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.
| ||
J Biol Chem Acute loss of iron-sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells. | ||
PLoS One Amino Acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis. |