GCOM1 Polyclonal antibody

GCOM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18129-1-AP

Synonyms

GRINL1A upstream protein, GRINL1A complex locus, GRINL1A combined protein, GRINL1A, Gcom2

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Validation Data Gallery

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  WB analysis using 18129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18129-1-AP (GCOM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18129-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18129-1-AP (GCOM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18129-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18129-1-AP (GCOM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18129-1-AP

  Immunofluorescent analysis of HepG2 cells, using GCOM1 antibody 18129-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18129-1-AP targets GCOM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GCOM1 fusion protein Ag12708
• Full Name: GRINL1A complex locus
• Calculated Molecular Weight: 466 aa, 54 kDa
• Observed Molecular Weight: 64 kDa, 44-54 kDa
• GenBank Accession Number: BC101645
• Gene Symbol: GCOM1
• Gene ID (NCBI): 145781
• RRID: AB_2232101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCOM1, also named as Myozap, Gup and Gcom, belongs to the GCOM1 family. It plays a role in cellular signaling via Rho-related GTP-binding proteins and subsequent activation of transcription factor SRF. GCOM1 has 11 isoforms with MW 44-54kd and 64kd.