Filter: AllWBIHCAffinity

WB analysis using 82623-1-RR (same clone as 82623-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 82623-1-RR (GCLM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 82623-1-RR (same clone as 82623-1-PBS)
WB result of GCLM antibody (82623-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HeLa cells. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.
WB analysis of RAW 264.7 using 82623-1-RR (same clone as 82623-1-PBS)
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 82623-1-RR (GCLM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 82623-1-RR (same clone as 82623-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82623-1-RR (GCLM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 82623-1-RR (same clone as 82623-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82623-1-RR (GCLM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.
IHC staining of human ovarian cancer using 82623-1-RR (same clone as 82623-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.
IHC staining of human ovarian cancer using 82623-1-RR (same clone as 82623-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 82623-1-RR (same clone as 82623-1-PBS)
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 82623-1-RR (same clone as 82623-1-PBS)
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 82623-1-RR
Biolayer interferometry (BLl) kinetic assays of 82623-1-RR against Human GCLM were performed. The affinity constant is below 1 pM.

Featured Product
KD/KO Validated

GCLM Recombinant antibody, PBS Only

GCLM Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 82623-1-PBS

Clone No.4K16

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GLCLR, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

82623-1-PBS targets GCLM in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	GCLM fusion protein Ag5497 Predict reactive species
Full Name	glutamate-cysteine ligase, modifier subunit
Calculated Molecular Weight	31 kDa
Observed Molecular Weight	31 kDa
GenBank Accession Number	BC041809
Gene Symbol	GCLM
Gene ID (NCBI)	2730
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P48507
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.