GCLC Polyclonal antibody

• Featured Product
• KD/KO Validated

GCLC Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

123

Reviews (1)

Cat no : 12601-1-AP

Synonyms

Gamma ECS, GCLC, GCS, GCS heavy chain, GLCL, GLCLC

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Validation Data Gallery

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  WB analysis using 12601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12601-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12601-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12601-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12601-1-AP

  IP result of anti-GCLC (IP:12601-1-AP, 4ug; Detection:12601-1-AP 1:5000) with mouse kidney tissue lysate 2160 ug.

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  IHC staining of human kidney using 12601-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12601-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 12601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 12601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse kidney tissue, L02 cells, HEK-293 cells, Raji cells, mouse lung tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 121 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Product Information

12601-1-AP targets GCLC in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GCLC fusion protein Ag3252
• Full Name: glutamate-cysteine ligase, catalytic subunit
• Calculated Molecular Weight: 637 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC022487
• Gene Symbol: GCLC
• Gene ID (NCBI): 2729
• RRID: AB_2278734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).

Publications

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang ; KO Validated
human	WB	Cell; Differential Requirements for eIF4E Dose in Normal Development and Cancer.; Authors - Morgan L Truitt
mouse	WB	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi
mouse	WB	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
mouse	WB	Cell Metab; Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.; Authors - Joni Nikkanen
mouse	WB	Hepatology; Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.; Authors - Yu Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

AIK SENG (Verified Customer) (01-10-2023)

Clear & crisp bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Colorectal cancer