GCHFR Polyclonal antibody

• Featured Product
• KD/KO Validated

GCHFR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18809-1-AP

Synonyms

GCHFR, GFRP, HsT16933, P35

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Validation Data Gallery

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  WB analysis using 18809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18809-1-AP (GCHFR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervix using 18809-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 18809-1-AP (GCHFR antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervix using 18809-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 18809-1-AP (GCHFR antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human cervix tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

18809-1-AP targets GCHFR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GTP cyclohydrolase I feedback regulator
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC112262
• Gene Symbol: GCHFR
• Gene ID (NCBI): 2644
• RRID: AB_10596912
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCHFR,also named as p35 and GFRP, belongs to the GFRP family. GCHFR mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase 1. It modulates BH4 synthesis and oxidative stress. This inhibition is reversed by L-phenylalanine.

Publications

Species	Application	Title
mouse	WB,IHC	Antioxid Redox Signal; Characterization of Transgenic Gfrp Knock-In Mice: Implications for Tetrahydrobiopterin in Modulation of Normal Tissue Radiation Responses.; Authors - Pathak Rupak R ; KO Validated