GCH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GCH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 28501-1-AP

Synonyms

DYT14, DYT5, GCH, GCH1, GTP CH 1, GTP CH I, GTP cyclohydrolase 1, GTP cyclohydrolase I, GTPCH1

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Validation Data Gallery

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  WB analysis using 28501-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28501-1-AP (GCH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 28501-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28501-1-AP (GCH1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 28501-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28501-1-AP (GCH1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 28501-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28501-1-AP (GCH1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 28501-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28501-1-AP (GCH1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: rat liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 2 publications below

Product Information

28501-1-AP targets GCH1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GCH1 fusion protein Ag28896
• Full Name: GTP cyclohydrolase 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC025415
• Gene Symbol: GCH1
• Gene ID (NCBI): 2643
• RRID: AB_2881157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Free Radic Res; 3,6-Epidioxy-1,10-bisaboladiene induces ferroptosis-like cell death through lipid peroxidation; Authors - Narandulam Usukhbayar
mouse,human	WB	Nat Cell Biol; Gut microbial metabolite facilitates colorectal cancer development via ferroptosis inhibition; Authors - Weiwei Cui
human	WB	Int J Oncol; Long non‑coding RNA lung cancer‑associated transcript 1 regulates ferroptosis via microRNA‑34a‑5p‑mediated GTP cyclohydrolase 1 downregulation in lung cancer cells; Authors - Fumin Tai
human,mouse	WB,IF	Neuron; Spliceosomal GTPase Eftud2 deficiency-triggered ferroptosis leads to Purkinje cell degeneration; Authors - Guochao Yang
	WB	Cell Death Dis; Identification of genes supporting cold resistance of mammalian cells: lessons from a hibernator; Authors - Masamitsu Sone
human	WB	Eur Rev Med Pharmacol Sci; Identification of ferroptosis-related genes for the prediction of prognosis and chemotherapy benefit of gastric cancer; Authors - Jiujian Zheng