GBP4 Recombinant antibody, PBS Only

GBP4 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240730H5

Cat no : 83699-4-PBS

Synonyms

Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4, EC:3.6.5.-

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Validation Data Gallery

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  WB analysis of HeLa using 83699-4-RR (same clone as 83699-4-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 83699-4-RR (GBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83699-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83699-4-RR (same clone as 83699-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GBP4 Recombinant antibody (83699-4-RR, Clone:240730H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83699-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83699-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83699-4-RR against Human GBP4 were performed. The affinity constant is 11.6 pM.

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Product Information

The immunogen of 83699-4-PBS is GBP4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GBP4 fusion protein Ag12006
• Full Name: guanylate binding protein 4
• Calculated Molecular Weight: 640 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC070055
• Gene Symbol: GBP4
• Gene ID (NCBI): 115361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Guanylate-binding protein 4 (GBP4) is an IFN-inducible GTPase that belongs to the GB1/RHD3 GTPase family. GBP4 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. GBP4 negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor. The molecular weight of GBP4 is 73 kDa.