GBP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

GBP2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 27299-1-AP

Synonyms

GBP 2, GBP2, GTP binding protein 2, HuGBP 2

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Validation Data Gallery

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  WB analysis using 27299-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27299-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27299-1-AP

  WB result of GBP2 antibody (27299-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBP2 transfected HeLa cells.

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  WB analysis of HeLa using 27299-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27299-1-AP (GBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 27299-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27299-1-AP (GBP2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 27299-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27299-1-AP (GBP2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A375 cells, HeLa cells, mouse spleen tissue, rat spleen tissue
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

27299-1-AP targets GBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GBP2 fusion protein Ag25433
• Full Name: guanylate binding protein 2, interferon-inducible
• Calculated Molecular Weight: 591 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC022272
• Gene Symbol: GBP2
• Gene ID (NCBI): 2634
• RRID: AB_2880836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Immunol; IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death.; Authors - Rajendra Karki
human	WB,IHC	Anticancer Res; Guanylate-binding Protein 2 Expression Is Associated With Poor Survival and Malignancy in Clear-cell Renal Cell Carcinoma.; Authors - Qingyuan Liu ; KD Validated
human	WB,IHC	Oncol Rep; GBP2 promotes clear cell renal cell carcinoma progression through immune infiltration and regulation of PD‑L1 expression via STAT1 signaling; Authors - Shujiang Ye