GBP2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

GBP2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11854-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 11854-1-AP (same clone as 11854-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

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  WB analysis using 11854-1-AP (same clone as 11854-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 11854-1-AP (same clone as 11854-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

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  WB analysis of human lung using 11854-1-AP (same clone as 11854-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 11854-1-AP (same clone as 11854-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 11854-1-AP (same clone as 11854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11854-1-AP (GBP2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 11854-1-AP (same clone as 11854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11854-1-AP (GBP2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 11854-1-AP (same clone as 11854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11854-1-AP (GBP2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 11854-1-AP (same clone as 11854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11854-1-AP (GBP2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11854-1-AP (same clone as 11854-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GBP2 antibody (11854-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11854-1-PBS in a different storage buffer formulation.

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Product Information

11854-1-PBS targets GBP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GBP2 fusion protein Ag2425
• Full Name: guanylate binding protein 2, interferon-inducible
• Calculated Molecular Weight: 591 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC022272
• Gene Symbol: GBP2
• Gene ID (NCBI): 2634
• RRID: AB_2109336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)