GBE1 Recombinant antibody

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  WB analysis using 85143-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85143-5-RR (GBE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85143-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85143-5-RR (GBE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

GBE1 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 85143-5-RR

Clone No.242705G3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Glycogen-branching enzyme, GBE, EC:2.4.1.18, APBD, 242705G3

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse lung tissue, rat lung tissue, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85143-5-RR targets GBE1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GBE1 fusion protein Ag14154
• Full Name: glucan (1,4-alpha-), branching enzyme 1
• Calculated Molecular Weight: 702 aa, 80 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC012098
• Gene Symbol: GBE1
• Gene ID (NCBI): 2632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q04446
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GBE1 (glucan (1,4-alpha-), branching enzyme 1) is essential for the globular and branched structure of glycogen, increasing solubility by creating a hydrophilic surface and reducing intracellular osmotic pressure. It may serve as a potential therapeutic target for treating LUAD(PMID: 31221150). The full sequence can be further processed into a mature form of 75 kDa.