GATM Polyclonal antibody

GATM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 12801-1-AP

Synonyms

AGAT, AT, GATM, Transamidinase

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Validation Data Gallery

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  WB analysis of mouse kidney using 12801-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12801-1-AP (GATM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12801-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12801-1-AP (GATM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 12801-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12801-1-AP (GATM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 12801-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12801-1-AP (GATM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 12801-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12801-1-AP (GATM antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human kidney tissue, rat kidney tissue, rat pancreas tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

12801-1-AP targets GATM in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GATM fusion protein Ag3754
• Full Name: glycine amidinotransferase (L-arginine:glycine amidinotransferase)
• Calculated Molecular Weight: 423 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC004141
• Gene Symbol: GATM
• Gene ID (NCBI): 2628
• RRID: AB_2109060
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATM, also known as AGAT, produces ornithine and guanidinoacetate (GAA) from arginine and glycine, and is a key enzyme for creatine synthesis. GATM deficiency in early infancy causes neurodevelopmental delay. GATM is predominantly expressed in kidney and pancreas in adults. Decrease of GAMT proteins was observed in proximal tubule damage cases, which links GATM as a general marker for proximal tubule damage. Alternative splicing of GATM generates three isoforms with molecular weights between 44 kDa to 48 kDa.

Publications

Species	Application	Title
mouse	WB,IHC	Biomed Pharmacother; Metabolomic profiling of metoprolol-induced cardioprotection in a murine model of acute myocardial ischemia.; Authors - Qiong Lai
mouse	WB	J Agric Food Chem; Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.; Authors - Sho Nishikawa
mouse	WB	Front Physiol; Arginine:Glycine Amidinotransferase Is Essential for Creatine Supply in Mice During Chronic Hypoxia.; Authors - Juliane Hannemann
mouse	WB	Front Nutr; Influence of homoarginine on creatine accumulation and biosynthesis in the mouse; Authors - Craig A. Lygate
mouse	WB	Amino Acids; Muscle phenotype of AGAT- and GAMT-deficient mice after simvastatin exposure.; Authors - Ali Sasani
human	IF	Gene; Clinical and functional characterization of rare compound heterozygous mutations in the SERPINC1 gene causing severe thrombophilia; Authors - Ke Zhang