GATC Monoclonal antibody

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  WB analysis of A549 using 68405-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68405-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 68405-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GATC antibody (68405-1-Ig, Clone: 2F9C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of HEK-293 using 68405-1-Ig

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GATC Monoclonal antibody (68405-1-Ig, Clone:2F9C8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

GATC Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68405-1-Ig

Clone No.2F9C8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Protein 15E1.2, Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial, Glu-AdT subunit C, GatC like protein, EC:6.3.5.-

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Tested Applications

• Positive WB detected in: LNCaP cells, A549 cells, HEK-293 cells, K-562 cells
• Positive IF/ICC detected in: HEK-293 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68405-1-Ig targets GATC in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GATC fusion protein Ag19857
• Full Name: glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial)
• Calculated Molecular Weight: 136 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC107145
• Gene Symbol: GATC
• Gene ID (NCBI): 283459
• RRID: AB_3085122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43716
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATC encodes a subunit of the glutamyl-tRNA(Gln) amidotransferase protein complex (GatCAB), which plays a role in aminoacylation of mitochondrial glutaminyl mt-tRNA. The complex plays an role in mitochondrial translation and protein synthesis (PubMed: 30283131).