GATA1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

GATA1 Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5E2A8

Cat no : 60011-1-PBS

Synonyms

ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT

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Validation Data Gallery

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  WB analysis using 60011-1-Ig (same clone as 60011-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 60011-1-Ig (same clone as 60011-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 60011-1-Ig (same clone as 60011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 60011-1-Ig (same clone as 60011-1-PBS)

  1X10^6 Jurkat cells were stained with 0.20ug GATA1 antibody (60011-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH. This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60011-1-PBS targets GATA1 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GATA1 fusion protein Ag1350
• Full Name: GATA binding protein 1 (globin transcription factor 1)
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC009797
• Gene Symbol: GATA1
• Gene ID (NCBI): 2623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].