GAT1 Recombinant antibody

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  WB analysis using 85060-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85060-2-RR (SLC6A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 85060-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 85060-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 85060-2-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85060-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85060-2-RR against Human GAT1 were performed. The affinity constant is below 1 pM.

GAT1 {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA

Cat No. 85060-2-RR

Clone No.242637D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SLC6A1, GAT 1, GABT1, GABATR, GABATHG

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Tested Applications

• Positive WB detected in: unboiled rat brain tissue
• Positive IHC detected in: mouse cerebellum tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85060-2-RR targets GAT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GAT1 fusion protein Ag29712
• Full Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 1
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC033904
• Gene Symbol: GABA Transporter 1/GAT1
• Gene ID (NCBI): 6529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30531
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.