GAS7 Polyclonal antibody

GAS7 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10072-1-AP

Synonyms

KIAA0394, Growth arrest-specific protein 7, GAS-7, GAS 7

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 10072-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10072-1-AP (GAS7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10072-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10072-1-AP (GAS7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 10072-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GAS7 antibody (10072-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, mouse testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

10072-1-AP targets GAS7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GAS7 fusion protein Ag0114
• Full Name: growth arrest-specific 7
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC001152
• Gene Symbol: GAS7
• Gene ID (NCBI): 8522
• RRID: AB_2108090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Growth arrest-specific (GAS) genes are expressed preferentially in cells that enter a quiescent state. Gas7, first identified in serum-starved murine fibroblasts, encodes a 48-kDa protein. 3 isoforms of Gas7 were identified in rat brains, with molecular weight of 38, 47 and 55 kDa in SDS Page.

Publications

Species	Application	Title
mouse	WB	Biomater Sci; Dicalcium silicate microparticles modulate the differential expression of circRNAs and mRNAs in BMSCs and promote osteogenesis via circ_1983-miR-6931-Gas7 interaction.; Authors - Wenchao Zhong
rat	WB,IHC	J Proteome Res; Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins.; Authors - Ji Yuhua Y
human	WB	J Cancer; The METTL3/miR-196a Axis Predicts Poor Prognosis in Non-small Cell Lung Cancer; Authors - Zhen Yang