GAS2L1 Monoclonal antibody, PBS Only

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  WB analysis using 68051-1-Ig (same clone as 68051-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68051-1-Ig (GAS2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 68051-1-Ig (same clone as 68051-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 68051-1-Ig (GAS2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68051-1-Ig (same clone as 68051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68051-1-Ig (GAS2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 68051-1-Ig (same clone as 68051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68051-1-Ig (GAS2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

GAS2L1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 68051-1-PBS

Clone No.2C6D5

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

GAR22, GAS2 like protein 1, GAS2L1, growth arrest 2 like 1

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Product Information

68051-1-PBS targets GAS2L1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GAS2L1 fusion protein Ag31232
• Full Name: growth arrest-specific 2 like 1
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC011047
• Gene Symbol: GAS2L1
• Gene ID (NCBI): 10634
• RRID: AB_2918793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q99501
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

GAS2L1(growth arrest‐specific 2‐like 1) is a component of the cytoskeleton that mediates the interaction between microtubules and microfilament (PMID: 34555082). GAS2L1 contains an N‐terminal actin‐binding calponin homology (CH) domain, a MT lattice‐binding Gas2‐related (GAR) domain and a C‐terminal SxIP motif, which mediates the interaction with MT plus ends via EB proteins. GAS2L1 regulates microtubule dynamics and stability by interacting with microtubule plus-end tracking proteins, such as MAPRE1, to regulate microtubule growth along actin stress fibers (PubMed:24706950).