GART Monoclonal antibody

GART Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A11E2

Reviews (1)

Cat no : 67939-1-Ig

Synonyms

AIR synthase, AIRS, GAR transformylase, GARS, GART, GARTF, PAIS, PGFT, PRGS

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Validation Data Gallery

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  WB analysis using 67939-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67939-1-Ig (GART antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67939-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67939-1-Ig (GART antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 67939-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GART antibody (67939-1-Ig, Clone: 2A11E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67939-1-Ig targets GART in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GART fusion protein Ag30509
• Full Name: phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
• Calculated Molecular Weight: 1010 aa, 108 kDa
• Observed Molecular Weight: 46 kDa, 108 kDa
• GenBank Accession Number: BC038958
• Gene Symbol: GART
• Gene ID (NCBI): 2618
• RRID: AB_2918691
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (11-11-2022)

Great antibody. Very strong signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: HepG2 cell/ Mouse liver

GART Antibody Western Blot validation (1:8000 dilution) in HepG2 cell/ Mouse liver (Cat no:67939-1-Ig)