GART Polyclonal antibody

• Featured Product
• KD/KO Validated

GART Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 13659-1-AP

Synonyms

GARTF, GARS, GAR transformylase, AIRS, AIR synthase

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Validation Data Gallery

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  WB analysis using 13659-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13659-1-AP (GART antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13659-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13659-1-AP (GART antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13659-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13659-1-AP (GART antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13659-1-AP

  IP result of anti-GART (IP:13659-1-AP, 4ug; Detection:13659-1-AP 1:500) with HepG2 cells lysate 1600ug.

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  IHC staining of human cervical cancer using 13659-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13659-1-AP (GART antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13659-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13659-1-AP (GART antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13659-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13659-1-AP (GART antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, HeLa cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

13659-1-AP targets GART in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GART fusion protein Ag4555
• Full Name: phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
• Calculated Molecular Weight: 1010 aa, 108 kDa
• Observed Molecular Weight: 108 kDa, 46 kDa
• GenBank Accession Number: BC038958
• Gene Symbol: GART
• Gene ID (NCBI): 2618
• RRID: AB_2107937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GART is a trifunctional polypeptide which has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity that is required for de novo purine biosynthesis. GART has 2 isoforms with molecular mass of 108 kDa and 46 kDa due to alternative splicing.

Publications

Species	Application	Title
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
mouse	WB	Nat Commun; Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway.; Authors - Mona Hoseini Soflaee
human	WB	EMBO J; UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism.; Authors - Xing Feng
human	WB	Cell Rep; Purine Nucleotide Availability Regulates mTORC1 Activity through the Rheb GTPase.; Authors - Natasha Emmanuel
human	WB	Nat Metab; Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.; Authors - Diem H Tran

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (08-26-2019)

Works well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HepG2, mouse liver, mouse lung, mouse hippocampus

GART Antibody Western Blot, validation (1:500 dilution) in HepG2, mouse liver, mouse lung, mouse hippocampus (Cat no:13659-1-AP)