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  • KD/KO Validated

GARS Polyclonal antibody

GARS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 15831-1-AP

Synonyms

AP 4 A synthetase, CMT2D, DSMAV, GARS, Glycine tRNA ligase, glycyl tRNA synthetase, GlyRS, HMN5, SMAD1



Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells, HepG2 cells, HT-1080 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15831-1-AP targets GARS in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, Drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GARS fusion protein Ag8322
Full Name glycyl-tRNA synthetase
Calculated Molecular Weight 739 aa, 83 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC007755
Gene Symbol GARS
Gene ID (NCBI) 2617
RRIDAB_2294385
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GARS(glycinamide ribonucleotide synthetase) perform an essential function in protein synthesis by catalyzing the esterification of an amino acid to its cognate tRNA.GARS, however, is a ubiquitously expressed α2 homodimer responsible for charging tRNA molecules with glycine, this enzyme is essential for protein synthesis in all cells and tissues(PMID:17035524 ).It can exist as a oligomer with 250KDa(PMID:22504945).

Protocols

Product Specific Protocols
WB protocol for GARS antibody 15831-1-APDownload protocol
IHC protocol for GARS antibody 15831-1-APDownload protocol
IF protocol for GARS antibody 15831-1-APDownload protocol
IP protocol for GARS antibody 15831-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseCoIP

Cell

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules

Authors - Qinqin Cui
humanWB

J Hematol Oncol

Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Authors - Ning Xu
mouseWB

Aging Cell

SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy.

Authors - Yingying Zhao
mouseWB,IF

Brain

HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease.

Authors - Veronick Benoy
  • KD Validated
DrosophilaWB

Nat Commun

Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases.

Authors - Sven Niehues
humanWB,RIP

EMBO Rep

The tRNA-like small noncoding RNA mascRNA promotes global protein translation.

Authors - Xinping Lu
  • KD Validated