GARS Polyclonal antibody

• Featured Product
• KD/KO Validated

GARS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 15831-1-AP

Synonyms

AP 4 A synthetase, CMT2D, DSMAV, GARS, Glycine tRNA ligase, glycyl tRNA synthetase, GlyRS, HMN5, SMAD1

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Validation Data Gallery

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  WB analysis of human brain using 15831-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15831-1-AP

  WB result of GARS antibody (15831-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARS transfected HepG2 cells.

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  WB analysis of HepG2 using 15831-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15831-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 15831-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 15831-1-AP (GARS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15831-1-AP

  IP result of anti-GARS (IP:15831-1-AP, 4ug; Detection:15831-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human skin cancer using 15831-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15831-1-AP (GARS Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 15831-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15831-1-AP (GARS Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HepG2 using 15831-1-AP

  Immunofluorescent analysis of HepG2 cells, using GARS antibody 15831-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human brain tissue, HeLa cells, HepG2 cells, HT-1080 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Product Information

15831-1-AP targets GARS in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, Drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GARS fusion protein Ag8322
• Full Name: glycyl-tRNA synthetase
• Calculated Molecular Weight: 739 aa, 83 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC007755
• Gene Symbol: GARS
• Gene ID (NCBI): 2617
• RRID: AB_2294385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GARS(glycinamide ribonucleotide synthetase) perform an essential function in protein synthesis by catalyzing the esterification of an amino acid to its cognate tRNA.GARS, however, is a ubiquitously expressed α2 homodimer responsible for charging tRNA molecules with glycine, this enzyme is essential for protein synthesis in all cells and tissues(PMID:17035524 ).It can exist as a oligomer with 250KDa(PMID:22504945).

Publications

Species	Application	Title
human,mouse	CoIP	Cell; Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules; Authors - Qinqin Cui
human	WB	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu
mouse	WB	Aging Cell; SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy.; Authors - Yingying Zhao
mouse	WB,IF	Brain; HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease.; Authors - Veronick Benoy ; KD Validated
Drosophila	WB	Nat Commun; Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases.; Authors - Sven Niehues
human	WB,RIP	EMBO Rep; The tRNA-like small noncoding RNA mascRNA promotes global protein translation.; Authors - Xinping Lu ; KD Validated