GAPVD1 Polyclonal antibody

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  WB analysis using 16940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16940-1-AP (GAPVD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16940-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16940-1-AP (GAPVD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16940-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16940-1-AP (GAPVD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 16940-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GAPVD1 antibody (16940-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

GAPVD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16940-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RAP6, Rab5-activating protein 6, KIAA1521, GTPase-activating protein and VPS9 domain-containing protein 1, GAPex-5

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16940-1-AP targets GAPVD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GAPVD1 fusion protein Ag10492
• Full Name: GTPase activating protein and VPS9 domains 1
• Calculated Molecular Weight: 1478 aa, 165 kDa
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: BC021119
• Gene Symbol: GAPVD1
• Gene ID (NCBI): 26130
• RRID: AB_3085511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14C86
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GTPase activating protein and VPS9 domains 1(GAPVD1) is a multifunctional protein involved in cellular processes such as endocytosis, insulin receptor internalization, and GLUT4 trafficking. Its function is critical in maintaining cellular homeostasis and it interacts with various proteins, including those involved in endocytosis and circadian rhythm regulation.